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The Intracellular Loop of the Na<sup>+</sup>/Ca<sup>2+</sup> Exchanger Contains an "Awareness Ribbon"-Shaped Two-Helix Bundle Domain

5 years 9 months ago
The Na^(+)/Ca^(2+) exchanger (NCX) is a ubiquitous single-chain membrane protein that plays a major role in regulating the intracellular Ca^(2+) homeostasis by the counter transport of Na^(+) and Ca^(2+) across the cell membrane. Other than its prokaryotic counterpart, which contains only the transmembrane domain and is self-sufficient as an active ion transporter, the eukaryotic NCX protein possesses in addition a large intracellular loop that senses intracellular calcium signals and controls...
Jiaqi Yuan

The Intracellular Loop of the Na<sup>+</sup>/Ca<sup>2+</sup> Exchanger Contains an "Awareness Ribbon"-Shaped Two-Helix Bundle Domain

5 years 9 months ago
The Na^(+)/Ca^(2+) exchanger (NCX) is a ubiquitous single-chain membrane protein that plays a major role in regulating the intracellular Ca^(2+) homeostasis by the counter transport of Na^(+) and Ca^(2+) across the cell membrane. Other than its prokaryotic counterpart, which contains only the transmembrane domain and is self-sufficient as an active ion transporter, the eukaryotic NCX protein possesses in addition a large intracellular loop that senses intracellular calcium signals and controls...
Jiaqi Yuan

(1)H, (13)C, (15)N resonance assignment of recombinant Euplotes raikovi protein Er-23

5 years 9 months ago
Er-23 is a small, 51 amino acid, disulfide-rich pheromone protein used for cell signaling by Euplotes raikovi. Ten of the 51 amino acids are cysteine, allowing up to five disulfide bonds. Previous NMR work with Er-23 utilized homologously expressed protein, prohibiting isotopic labeling, and consequently the chemical shift assignments were incomplete. We have expressed uniformly ^(15)N and ^(13)C-labeled Er-23 in an E. coli expression system. Here we report the full backbone and side chain...
David P Bowles

(1)H, (13)C, (15)N resonance assignment of recombinant Euplotes raikovi protein Er-23

5 years 9 months ago
Er-23 is a small, 51 amino acid, disulfide-rich pheromone protein used for cell signaling by Euplotes raikovi. Ten of the 51 amino acids are cysteine, allowing up to five disulfide bonds. Previous NMR work with Er-23 utilized homologously expressed protein, prohibiting isotopic labeling, and consequently the chemical shift assignments were incomplete. We have expressed uniformly ^(15)N and ^(13)C-labeled Er-23 in an E. coli expression system. Here we report the full backbone and side chain...
David P Bowles

Histogram analysis of apparent diffusion coefficient from whole-body diffusion-weighted MRI to predict early response to chemotherapy in patients with metastatic colorectal cancer: preliminary results

5 years 10 months ago
CONCLUSION: ADC histogram analysis shows potential in discriminating progressive from non-progressive disease in patients with mCRC, who underwent whole-body DW-MRI. The technique can potentially be tested as a response assessment methodology in larger trials.
I Lavdas

Meeting – April 23rd @ 5:30PM

5 years 11 months ago
Prof. Lars Konnerman, The University of Western Ontario, London “From Solution into the Gas Phase: A Look at Electrosprayed Protein Ions and ESI Mechanisms” Date: April 23rd Time: 5:30PM Location: TBA
hall.1443

Meeting – March 29th @ 5:30PM

6 years ago
Prof. Laura Reed, University of Alabama, Tuscaloosa “Disentangling the gene-by-environment interaction architecture of metabolic disease through the lens of evolutionary genetics and metabolomics” Date: March 29th Time: 5:30PM Location: TBA
hall.1443

Non-Uniform and Absolute Minimal Sampling for High-Throughput Multidimensional NMR Applications

6 years ago
Many biomolecular NMR applications can benefit from the faster acquisition of multidimensional NMR data with high resolution and their automated analysis and interpretation. In recent years, a number of non-uniform sampling (NUS) approaches have been introduced for the reconstruction of multidimensional NMR spectra, such as compressed sensing, thereby bypassing traditional Fourier-transform processing. Such approaches are applicable to both biomacromolecules and small molecules and their complex...
Dawei Li

Non-Uniform and Absolute Minimal Sampling for High-Throughput Multidimensional NMR Applications

6 years ago
Many biomolecular NMR applications can benefit from the faster acquisition of multidimensional NMR data with high resolution and their automated analysis and interpretation. In recent years, a number of non-uniform sampling (NUS) approaches have been introduced for the reconstruction of multidimensional NMR spectra, such as compressed sensing, thereby bypassing traditional Fourier-transform processing. Such approaches are applicable to both biomacromolecules and small molecules and their complex...
Dawei Li

Nanoparticle-Assisted Metabolomics

6 years ago
Understanding and harnessing the interactions between nanoparticles and biological molecules is at the forefront of applications of nanotechnology to modern biology. Metabolomics has emerged as a prominent player in systems biology as a complement to genomics, transcriptomics and proteomics. Its focus is the systematic study of metabolite identities and concentration changes in living systems. Despite significant progress over the recent past, important challenges in metabolomics remain, such as...
Bo Zhang

Nanoparticle-Assisted Metabolomics

6 years ago
Understanding and harnessing the interactions between nanoparticles and biological molecules is at the forefront of applications of nanotechnology to modern biology. Metabolomics has emerged as a prominent player in systems biology as a complement to genomics, transcriptomics and proteomics. Its focus is the systematic study of metabolite identities and concentration changes in living systems. Despite significant progress over the recent past, important challenges in metabolomics remain, such as...
Bo Zhang

Meeting – February 20th @ 5:30PM

6 years 1 month ago
Mr. William Andrews, Hummon Research Group, Ohio State “Enhancing Mass Spectrometric Molecular Imaging Through Active Analyte Extraction” Ms. Sierra Jackson, Badu Research Group, Ohio State “Thread Spray Mass Spectrometry for Direct Analysis of Capsaicinoids in Pepper Products” Date: February 20th Time: 5:30PM Location: TBA
hall.1443

Meeting – January 23rd @ 5:30PM

6 years 2 months ago
Mrs. Bernice Agana, Wysocki Research Group, Ohio State “Multi-omics Profiling of LKB1 Mutant NSCLC Cells to Characterize Deregulated Pathways Contributing to Tumorigenesis” Mr. Dmytro Kulyk, Badu Research Group, Ohio State “Multi-mode Nano-Contained Ion Source Capable of ESI and APCI” Date: January 23rd Time: 5:30PM Location: TBA
hall.1443

Reprograming of Glucose Metabolism by Zerumbone Suppresses Hepatocarcinogenesis

6 years 3 months ago
Hepatocellular carcinoma (HCC) is the most prevalent and highly aggressive liver malignancy with limited therapeutic options. Here, the therapeutic potential of zerumbone, a sesquiterpene derived from the ginger plant Zingiber zerumbet, against HCC was explored. Zerumbone inhibited proliferation and clonogenic survival of HCC cells in a dose-dependent manner by arresting cells at the G(2)-M phase and inducing apoptosis. To elucidate the underlying molecular mechanisms, a phosphokinase array was...
Nissar Ahmad Wani

Reprograming of Glucose Metabolism by Zerumbone Suppresses Hepatocarcinogenesis

6 years 3 months ago
Hepatocellular carcinoma (HCC) is the most prevalent and highly aggressive liver malignancy with limited therapeutic options. Here, the therapeutic potential of zerumbone, a sesquiterpene derived from the ginger plant Zingiber zerumbet, against HCC was explored. Zerumbone inhibited proliferation and clonogenic survival of HCC cells in a dose-dependent manner by arresting cells at the G(2)-M phase and inducing apoptosis. To elucidate the underlying molecular mechanisms, a phosphokinase array was...
Nissar Ahmad Wani

Bioassay-Guided Isolation of Antioxidant and Cytoprotective Constituents from a Maqui Berry (Aristotelia chilensis) Dietary Supplement Ingredient As Markers for Qualitative and Quantitative Analysis

6 years 6 months ago
Bioassay-guided phytochemical investigation of a commercially available maqui berry (Aristotelia chilensis) extract used in botanical dietary supplement products led to the isolation of 16 compounds, including one phenolic molecule, 1, discovered for the first time from a natural source, along with several known compounds, 2-16, including three substances not reported previously in A. chilensis, 2, 14, and 15. Each isolate was characterized by detailed analysis of NMR spectroscopic and HRESIMS...
Jie Li

Bioassay-Guided Isolation of Antioxidant and Cytoprotective Constituents from a Maqui Berry (Aristotelia chilensis) Dietary Supplement Ingredient As Markers for Qualitative and Quantitative Analysis

6 years 6 months ago
Bioassay-guided phytochemical investigation of a commercially available maqui berry (Aristotelia chilensis) extract used in botanical dietary supplement products led to the isolation of 16 compounds, including one phenolic molecule, 1, discovered for the first time from a natural source, along with several known compounds, 2-16, including three substances not reported previously in A. chilensis, 2, 14, and 15. Each isolate was characterized by detailed analysis of NMR spectroscopic and HRESIMS...
Jie Li

Fully automatic, multiorgan segmentation in normal whole body magnetic resonance imaging (MRI), using classification forests (CFs), convolutional neural networks (CNNs), and a multi-atlas (MA) approach

6 years 7 months ago
CONCLUSIONS: Three state-of-the-art algorithms were developed and used to automatically segment major organs and bones in whole body MRI; good agreement to manual segmentations performed by clinical MRI experts was observed. CNNs perform favorably, when using T2w volumes as input. Using multimodal MRI data as input to CNNs did not improve the segmentation performance.
Ioannis Lavdas

Maximal clique method for the automated analysis of NMR TOCSY spectra of complex mixtures

6 years 9 months ago
Characterization of the chemical components of complex mixtures in solution is important in many areas of biochemistry and chemical biology, including metabolomics. The use of 2D NMR total correlation spectroscopy (TOCSY) experiments has proven very useful for the identification of known metabolites as well as for the characterization of metabolites that are unknown by taking advantage of the good resolution and high sensitivity of this homonuclear experiment. Due to the complexity of the...
Da-Wei Li