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Dr. Damien Wilburn, Searle Research Group, Ohio State Comprehensive Cancer Center “Deep learning of proteome chemistry through harmonization of massive peptide libraries” Date: Feb 22nd, 2022 Time: 5:30 PM EST Zoom: https://osu.zoom.us/j/96434729214?pwd=R0U1K3hyZ2M1a1ZYR296eVRLcTVlZz09 Meeting ID: 964 3472 9214 Password: 859213

Ms. Elaura Gustafson, Brigham Young University, Daniel Austin’s Research Group “Printed Circuit Board Charge Detection Mass Spectrometry” Date: Jan 25th, 2022 Time: 5:30 PM EST Zoom: https://osu.zoom.us/j/96434729214?pwd=R0U1K3hyZ2M1a1ZYR296eVRLcTVlZz09 Meeting ID: 964 3472 9214 Password: 859213

Prof. Brian Searle, Biomedical Informatics, Ohio State Comprehensive Cancer Center Date: Dec 14th, 2021 Time: 5:30 PM EST Zoom: https://osu.zoom.us/j/96220959486?pwd=Y2R2K2EwUTArTXZ4b1IrT21DVXJKQT09 Meeting ID: 962 2095 9486 Password: 748441 Slides: “Designing DIA Experiments for Phosphoproteomics“

Chen Du (Wysocki Group) and Philip Lindhorst (Hummon Group) Date: Nov 16th, 2021 Time: 5:30 PM EST Zoom: https://osu.zoom.us/j/96220959486?pwd=Y2R2K2EwUTArTXZ4b1IrT21DVXJKQT09 Meeting ID: 962 2095 9486 Password: 748441

Title: “Exhalomics – on-line medical diagnosis via mass spectrometric analysis of exhaled breath” On-line breath analysis is a powerful approach to obtain insight into the metabolism of a person in real time. With ambient ionization methods, this can be achieved rapidly and completely non-invasively, opening up interesting possibilities to diagnose diseases via exhaled breath, to […]

Prof. Ekin Atilla-Gokcumen, University at Buffalo “LC-MS-based approaches for the discovery of novel lipid regulators of cell death” Date: Oct 12th, 2021 Time: 5:30 PM EST Zoom: https://osu.zoom.us/j/96220959486?pwd=Y2R2K2EwUTArTXZ4b1IrT21DVXJKQT09 Meeting ID: 962 2095 9486 Password: 748441

Dear All: Please consider registering for the upcoming MANA and OMSS symposia, to be held virtually in October 2021. You can find information about the program here. https://u.osu.edu/mana2021/

Mr. Taghi Sahraeian, Badu Research Group “Droplet Imbibition Mass Spectrometry” Date: Septh 14th, 2021 Time: 5:30 PM EST Zoom: link provided via email or contact badu-tawiah.1@osu.edu

The analysis of nuclear magnetic resonance (NMR) spectra for the comprehensive and unambiguous identification and characterization of peaks is a difficult, but critically important step in all NMR analyses of complex biological molecular systems. Here, we introduce DEEP Picker, a deep neural network (DNN)-based approach for peak picking and spectral deconvolution which semi-automates the analysis of two-dimensional NMR spectra. DEEP Picker includes 8 hidden convolutional layers and was trained...

The analysis of nuclear magnetic resonance (NMR) spectra for the comprehensive and unambiguous identification and characterization of peaks is a difficult, but critically important step in all NMR analyses of complex biological molecular systems. Here, we introduce DEEP Picker, a deep neural network (DNN)-based approach for peak picking and spectral deconvolution which semi-automates the analysis of two-dimensional NMR spectra. DEEP Picker includes 8 hidden convolutional layers and was trained...

Title: “Fishing out elusive species from chemical reactions” Prof. Shibdas Banerjee will be giving a talk at CBC’s analytical/physical seminar series. Shibdas is currently an Assistant Professor at Indian Institute of Science Education and Research Tirupati. He recently completed his postdoctoral studies with Prof. Dick Zare at Stanford University. Date: August 30th, 2021 Time: 4:10 […]

Amino-acid side-chain properties in proteins are key determinants of protein function. NMR spin relaxation of side chains is an important source of information about local protein dynamics and flexibility. However, traditional solution NMR relaxation methods are most sensitive to sub-nanosecond dynamics lacking information on slower ns-μs time-scale motions. Nanoparticle-assisted NMR spin relaxation (NASR) of methyl-side chains is introduced here as a window into these ns-μs dynamics. NASR...

Amino-acid side-chain properties in proteins are key determinants of protein function. NMR spin relaxation of side chains is an important source of information about local protein dynamics and flexibility. However, traditional solution NMR relaxation methods are most sensitive to sub-nanosecond dynamics lacking information on slower ns-μs time-scale motions. Nanoparticle-assisted NMR spin relaxation (NASR) of methyl-side chains is introduced here as a window into these ns-μs dynamics. NASR...

The sensitivity-enhanced HSQC, as well as HSQC-TOCSY, experiments have been modified for incorporation into NOAH (NMR by Ordered Acquisition using ¹H detection) supersequences, adding diversity for ^(13)C and ^(15)N modules. Importantly, these heteronuclear modules have been specifically tailored to preserve the magnetisation required for subsequent acquisition of other heteronuclear or homonuclear modules in a supersequence. In addition, we present protocols for optimally combining HSQC and...

The sensitivity-enhanced HSQC, as well as HSQC-TOCSY, experiments have been modified for incorporation into NOAH (NMR by Ordered Acquisition using ¹H detection) supersequences, adding diversity for ^(13)C and ^(15)N modules. Importantly, these heteronuclear modules have been specifically tailored to preserve the magnetisation required for subsequent acquisition of other heteronuclear or homonuclear modules in a supersequence. In addition, we present protocols for optimally combining HSQC and...

Two limiting cases of molecular recognition, induced fit (IF) and conformational selection (CS), play a central role in allosteric regulation of natural systems. The IF paradigm states that a substrate "instructs" the host to change its shape after complexation, while CS asserts that a guest "selects" the optimal fit from an ensemble of preexisting host conformations. With no studies that quantitatively address the interplay of two limiting pathways in abiotic systems, we herein and for the...

Two limiting cases of molecular recognition, induced fit (IF) and conformational selection (CS), play a central role in allosteric regulation of natural systems. The IF paradigm states that a substrate "instructs" the host to change its shape after complexation, while CS asserts that a guest "selects" the optimal fit from an ensemble of preexisting host conformations. With no studies that quantitatively address the interplay of two limiting pathways in abiotic systems, we herein and for the...

Sensitivity-improved versions of two-dimensional (2D) ^(13)C-¹H HSQC (heteronuclear single quantum coherence) and HSQC-TOCSY (HSQC-total correlation spectroscopy) NMR experiments optimized for small biological molecules and their complex mixtures encountered in metabolomics are presented that preserve the magnetization of ¹H spins not directly attached to ^(13)C spins. This allows (i) the application of rapid acquisition techniques to substantially shorten measurement time and (ii) their...

Sensitivity-improved versions of two-dimensional (2D) ^(13)C-¹H HSQC (heteronuclear single quantum coherence) and HSQC-TOCSY (HSQC-total correlation spectroscopy) NMR experiments optimized for small biological molecules and their complex mixtures encountered in metabolomics are presented that preserve the magnetization of ¹H spins not directly attached to ^(13)C spins. This allows (i) the application of rapid acquisition techniques to substantially shorten measurement time and (ii) their...

Prof. Justin Benesch, University of Oxford “Combining native mass spectrometry with mass photometry to elucidate the assembly of ultra-heterogeneous protein assemblies” Date: March 23rd, 2021 Time: 5:30 PM EST Zoom: link provided via email or contact badu-tawiah.1@osu.edu