Aggregator

Fred Strathman, Sr. VP of US Business, MOBILion Systems “The Duality of Ion Mobility: From Practical Applications to Novel Insights” Date: Friday, April 19, 2024 Time: 12:30pm Location: James L045

Dr. John Sausen, Agilent Technologies, Director of Strategic Initiatives — Mass Spectrometry “Advancing Ion Mobility into Lipidomics, Metabolomics, Exposomics, and Protein Characterization” Date: Friday, April 5, 2024 Time: 12:30pm Location: 014 University Hall Abstract Laboratories conducting Multi-Omics analysis have challenging requirements to better define isomeric and isoberic structures across a broad mass range in a […]

Ms. Kristie Baker, Wysocki Group, Ohio State “Characterizing a Surface Bridge in the Four-Helix Bundle Rop by Surface- and Collision-Induced Activation” and Philip Lacey, Wysocki Group, Ohio State “Heterogeneous Non-Canonical Inflammasome Oligomerization Probed by Narrow Quadrupole Selections and Electron Capture Charge Reduction” Date: Friday, February 23, 2024 Time: 12:30pm Location: 0035 Hitchcock Hall

Mr. Alex Grooms, Badu Group, Ohio State “Uncatalyzed Hydrazine Cross Coupling via Plasma-Microdroplet Fusion Enables Programmable C–N Bond Formation” Date: January 26, 2024 Time: 12:30 PM Location 0035 Hitchcock Hall

Despite rapid progress in metabolomics research, a major bottleneck is the large number of metabolites whose chemical structures are unknown or whose spectra have not been deposited in metabolomics databases. Nuclear magnetic resonance (NMR) spectroscopy has a long history of elucidating chemical structures from experimentally measured ¹H and ^(13)C chemical shifts. One approach to characterizing the chemical structures of an unknown metabolite is to predict the ¹H and ^(13)C chemical shifts of...

Despite rapid progress in metabolomics research, a major bottleneck is the large number of metabolites whose chemical structures are unknown or whose spectra have not been deposited in metabolomics databases. Nuclear magnetic resonance (NMR) spectroscopy has a long history of elucidating chemical structures from experimentally measured ¹H and ^(13)C chemical shifts. One approach to characterizing the chemical structures of an unknown metabolite is to predict the ¹H and ^(13)C chemical shifts of...

Ms. Ayesha Seth, Badu Group, Ohio State “Revolutionizing Malaria Detection: Pioneering Chemical Signal Amplification for Asymptomatic Cases with Mass Spectrometry” and Mr. Joseph Holbrook, Hummon Group, Ohio State “Enhancement of Lipid Signals in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry with Ammonium Fluoride as a Matrix Additive” Date: Dec 12, 2023 Time: 5:30 PM Location: BRT 105

Ms. Ariana Shannon (Hummon/Searle Group) “Generating fit-for-purpose targeted mass-spectrometry based proteomics assays from a global proteome catalog” Date: Nov 14, 2023 (TODAY) Time: 5:30 PM Location: BRT 105

Human K-Ras protein, which is a member of the GTPase Ras family, hydrolyzes GTP to GDP and concomitantly converts from its active to its inactive state. It is a key oncoprotein, because several mutations, particularly those at residue position 12, occur with a high frequency in a wide range of human cancers. The K-Ras protein is therefore an important target for developing therapeutic anti-cancer agents. In this work we report the almost complete sequence-specific resonance assignments of...

Human K-Ras protein, which is a member of the GTPase Ras family, hydrolyzes GTP to GDP and concomitantly converts from its active to its inactive state. It is a key oncoprotein, because several mutations, particularly those at residue position 12, occur with a high frequency in a wide range of human cancers. The K-Ras protein is therefore an important target for developing therapeutic anti-cancer agents. In this work we report the almost complete sequence-specific resonance assignments of...

The quantitative deconvolution of 1D-NMR spectra into individual resonances or peaks is a key step in many modern NMR workflows as it critically affects downstream analysis and interpretation. Depending on the complexity of the NMR spectrum, spectral deconvolution can be a notable challenge. Based on the recent deep neural network DEEP Picker and Voigt Fitter for 2D NMR spectral deconvolution, we present here an accurate, fully automated solution for 1D-NMR spectral analysis, including peak...

The quantitative deconvolution of 1D-NMR spectra into individual resonances or peaks is a key step in many modern NMR workflows as it critically affects downstream analysis and interpretation. Depending on the complexity of the NMR spectrum, spectral deconvolution can be a notable challenge. Based on the recent deep neural network DEEP Picker and Voigt Fitter for 2D NMR spectral deconvolution, we present here an accurate, fully automated solution for 1D-NMR spectral analysis, including peak...

Dr. Joshua Gilbert, Wysocki Research Group, Ohio State “Combining Soft-Landing MS and Low-Energy Electron Holography for Structural Biology” Date: October 24, 2023 Time: 5:30 PM Location: BRT 105

We describe the preparation, dynamic, assembly characteristics of vase-shaped basket 1^(3-) along with its ability to form an inclusion complex with anticancer drug mitoxantrone in abiotic and biotic systems. This novel cavitand has a deep nonpolar pocket consisting of three naphthalimide sides fused to a bicyclic platform at the bottom while carrying polar glycines at the top. The results of ¹ H Nuclear Magnetic Resonance (NMR), ¹ H NMR Chemical Exchange Saturation Transfer (CEST), Calorimetry,...

We describe the preparation, dynamic, assembly characteristics of vase-shaped basket 1^(3-) along with its ability to form an inclusion complex with anticancer drug mitoxantrone in abiotic and biotic systems. This novel cavitand has a deep nonpolar pocket consisting of three naphthalimide sides fused to a bicyclic platform at the bottom while carrying polar glycines at the top. The results of ¹ H Nuclear Magnetic Resonance (NMR), ¹ H NMR Chemical Exchange Saturation Transfer (CEST), Calorimetry,...

Mr. Alex Grooms, Badu Lab, Ohio State “Programmable Microdroplet Cascade Reactions: Uncatalyzed Michael Addition for Carbon-Carbon Bond Formation via Plasma-Water Fusion” and Mr. Daniel Quiroz Moreno (Cooperstone Group) “Lessons Learned from Data Dependent Acquisition and Molecular Networking: Case Application in Phenolics” Date: September 19, 2023 Time: 5:30 PM Location: BRT 105

An NMR NOAH-supersequence is presented consisting of five CEST experiments for studying protein backbone and side-chain dynamics by ^(15)N-CEST, carbonyl-^(13)CO-CEST, aromatic-^(13)C(ar)-CEST, ^(13)C(α)-CEST, and methyl-^(13)C(met)-CEST. The new sequence acquires the data for these experiments in a fraction of the time required for the individual experiments, saving over four days of NMR time per sample.

An NMR NOAH-supersequence is presented consisting of five CEST experiments for studying protein backbone and side-chain dynamics by ^(15)N-CEST, carbonyl-^(13)CO-CEST, aromatic-^(13)C(ar)-CEST, ^(13)C(α)-CEST, and methyl-^(13)C(met)-CEST. The new sequence acquires the data for these experiments in a fraction of the time required for the individual experiments, saving over four days of NMR time per sample.

Mr. Alex Grooms, Badu Lab, Ohio State “Dual Tunability for Uncatalyzed N-alkylation of Primary Amines Enabled by Super Reactive Electrospray Droplets” and Mr. Brian Fries, Hummon Lab, Ohio State “Fatty Acid Synthase Inhibition in Colorectal Cancer Spheroids” Date: April 18, 2023 Time: 5:30 PM Location: DHLRI 170

Dr. Marius Kostelic, Wysocki Lab, Ohio State “Architecture of Adeno-Associated Viral Capsids with Surface-Induced Dissociation and Charge Detection Mass Spectrometry” and Mr. Daniel Quiroz Moreno, Cooperstone Lab, Ohio State “MS2extract: An R Package for a Scalable MS/MS Compound Library Creation” Date: March 28, 2023 Time: 5:30 PM EST Location: DHLRI 170