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Mass Spec and Proteomics > Rates & Forms

Please choose the correct electronic form below based upon your affiliation to OSU:

On-Campus
Off-Campus Consortium (Non-OSU Ohio Universities)
Academic/Non-profit (Outside OH Consortium)
Off-Campus Industry

 

Not sure where to begin? Please use this guide to help you through the sample submission process!

 

Please Note - Electronic Service Forms

Please only submit service requests when you are ready to submit/send samples.

For External Users:
          Directions to submit a Service Request Form and samples to MS&P Faclity

          OSU Sponsored Guest Account / FOM Profile and Account Form

FACILITY ONLINE MANAGER (FOM) http://fom.osu.edu
Go to FOM to: add/inactivate existing account information, change profile information, view your Forms submitted to MS&P Facility, view billing information.

Sample Submission:

When submitting multiple samples, please fill out only one (1) Service Request Form in FOM and attach all sample information in a separate document. Please do not submit multiple forms. If you run into problems with FOM please contact fom-admin@osu.edu.


FY2020 BILLING RATES (Effective August 21st, 2019)

 
Unit OSU/
Consortium
* includes NCH
Academic/Non-profit (Outside OH Consortium) Rates Industry
Molecular weight/Chemical Formula determination
Accurate mass (FT-ICR and XL) Sample $50.00 $90.00 $170.00
Nominal mass (Amazon, UltrafleXtreme (TOF-TOF) Sample $40.00 $70.00 $110.00
Metabolomics
Method development
Qualitative/untargeted (LC-MS) Project $1000.00 $1500.00 $2000.00
Quantitative/targeted (LC-MS) Project $1200.00 $1800.00 $2500.00
Sample Analysis
LC-MS injection (< 10 samples, each injection), quantitative Injection $120.00 $180.00 $280.00
LC-MS injection (>10 samples, each injection), quantitative Injection $100.00 $150.00 $250.00
LC-MS injection (< 10 samples, each injection), qualitative Injection $140.00 $280.00 $350.00
LC-MS injection (>10 samples, each injection), qualitative Injection $120.00 $200.00 $300.00
Imaging/Tissue
Large molecules (>10,000 Da) MALDI TOF-TOF Sample $150.00 $500.00 $800.00
Small molecules (<10,000 Da), high resolution FT-ICR Sample $300.00 $550.00 $900.00
Self operation and Staff time (per hour)
Self operation Amazon (min 1/2 hr increments) Hour $5.00 $15.00 $50.00
Self operation MALDI TOF-TOF, Quantiva (min 1/2 hr increments) Hour $15.00 $30.00 $80.00
Self operation Orbis, Fusion, ICR (min 1/2 hr increments) Hour $20.00 $50.00 $120.00
Staff time Hour $50.00 $100.00 $200.00
Proteomics
1D SDS-PAGE - 10 Wells Sample $60.00 $100.00 $200.00
Peptide/Protein Enrichment, e.g., Phosphorylation Sample $50.00 $80.00 $120.00
Cell Lysis Sample $50.00 $80.00 $120.00
Coommassie Stain (Includes Image Acquisition) Gel $60.00 $120.00 $300.00
Enzyme Digestion Digestion $30.00 $50.00 $80.00
Protein Precipitation Sample $20.00 $40.00 $100.00
Protein Quantitation (Bradford), Peptide desalting Sample $20.00 $40.00 $100.00
Cryomill Sample $0.00 $20.00 $50.00
Lyophylizer Sample $0.00 $10.00 $20.00
Sample Analysis
LC-MS/MS Peptide Sequencing - Long Gradient (> 2hrs) - Fusion/QE Sample $250.00 $350.00 $800.00
LC-MS/MS Peptide Sequencing - Reg Gradient (2hrs) - Fusion/QE Sample $200.00 $280.00 $350.00
LC-MS/MS Peptide Sequencing - Reg Gradient (1hrs) - Fusion/QE Sample $150.00 $220.00 $280.00
LC-MS/MS - 2D or off line LC Fractions (per fraction) - Fusion/QE Fraction $100.00 $180.00 $300.00
Top Down Proteomics (Fusion, QE or ICR) Sample $500.00 $800.00 $1500.00
tims TOF Pro bottom up Sample $300.00 $400.00 $600.00

Sample Submission Guidelines


Samples can be brought to 250 Biomedical Research Tower, along with a filled out sample submission form, weekdays between 8 am and 5 pm. We have a refrigerator, freezer and dry box for samples that require special storage. It is preferred if samples are submitted neat or dried down, typically ug amounts is all that is needed.

Biological samples are handled differently and require different conditions. Salts, buffers and surfactants suppress ionization and need to be removed for mass spec analysis. When possible, it is advised to dialyze the sample into nanopure or at least distilled water prior to submission. We also have several de-salting techniques for proteins and oligonucleotides available in our facility. List all salts, buffers and detergents the protein or oligonucleotide is in, or has recently been in. For optimal results, please discuss your project with Dr. Somogyi prior to submission. Certain classes of proteins need to be handled differently than others, the more we know about your system, the better the data. Protein samples for ESI are prepared in 50% acetonitrile 50% water with 0.1% formic acid. Slightly acidic conditions are necessary to facilitate protonation

We CANNOT run samples that are in aromatic solvents like benzene, toluene, or solvents such as DMSO and DMF.

Electrospray and MALDI samples are prepared in Methanol, Acetonitrile, water, THF, chloroform. We will acidify the sample with acetic or formic acid unless you indicate the sample is acid sensitive.

Turn around times vary between 1 day to a week depending on sample load, instrument availability and type of analysis. We will email you when the results are ready to be picked up.